Western Blot:Article Title: Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis.
Article Snippet: Antibody(RRID) Vendor Catalog number Application Working Dilution for WB or (IF/IHC) Rabbit anti-Wilms Tumor1 (AB_2043201) Abcam Cambridge, MA,USA Ab89901 IF (1:200) Rabbit anti-ACTA2 (AB 2721021) ABclonal Shanghai, PRC A7248 IF (1:200) Rabbit anti-Raptor (AB 185538) Bethyl Montgomery,Alabama,USA A300–506A WB,IF 1:3000 (1:200) Rabbit anti-Vimentin (AB 1663663) Bioworld Louis Park, MN,USA BS1491 IF (1:200) Rabbit anti-Rheb (AB 2721022) Cell Signaling Technology Shanghai, PRC 13 879 WB 1:1000 Rabbit anti-Ki67 (AB 2687446) Cell Signaling Technology Shanghai, PRC 9129 IF (1:100) Rabbit anti-β-catenin (AB 331149) Cell Signaling Technology Shanghai, PRC 9562 IF (1:200) Rabbit anti-ZO1 (AB 10898025) Cell Signaling Technology Shanghai, PRC 8193 IF (1:100) Rabbit anti-ZO2 (AB 2203575) Cell Signaling Technology Shanghai, PRC 2847 IF (1:100) Rabbit anti-p-mTOR (AB 330970) Cell Signaling Technology Shanghai, PRC 2971 WB 1:1000 Rabbit anti-mTOR (AB 2105622) Cell Signaling Technology Shanghai, PRC 2983 WB 1:1000 Rabbit anti-p-p70S6K (AB 330944) Cell Signaling Technology Shanghai, PRC 9205 WB 1:2000 Rabbit anti-p70S6K (AB 390722) Cell Signaling Technology Shanghai, PRC 2708 WB 1:1000 Rabbit anti-p-S6 (AB 331679) Cell Signaling Technology Shanghai, PRC 2211 WB 1:3000 Mouse anti-S6 (AB 2238583) Cell Signaling Technology Shanghai, PRC 2317 WB 1:3000 Rabbit anti-ARHGEF4 (AB 2721023) Proteintech Rosemont,IL,USA 55 213–1-AP WB 1:2000 Rabbit anti-Cingulin (AB 10733647) Proteintech Rosemont,IL,USA 21 369–1-AP IF (1:200) Rabbit anti-PRM2 (AB 2721024) Proteintech Rosemont,IL,USA 14 500–1-AP WB 1:1000 Rabbit anti-TNP1 (AB 2206757) Proteintech Rosemont,IL,USA 17 178–1-AP WB 1:1000 Mouse anti-COL1A2 (AB 2716872) Santa Cruz Biotechnolog Dallas, TX,USA SC-393 573 IF (1:100) Mouse anti-Laminin α2 (AB 1125089) Santa Cruz Biotechnolog Dallas, TX,USA SC-55 605 IF (1:100) Mouse anti-β-Actin (AB 476697) Sigma-Aldrich St. Louis, MO,USA A2228 IF (1:200) Mouse anti-α-Tubulin (AB 477582) Sigma-Aldrich St. Louis, MO,USA T6074 IF (1:200) Mouse anti-GAPDH (AB 2721026) Tianjin Sungene Biotech Tianjin, PRC KM9002 WB 1:6000 Lectin PNA, Alexa Fluor TM 488 Conjugate (AB 2315178) Thermo Fisher Scientific L21409 IF 1:100 WB, western blot; IF, immunofluorescence; IHC, immunohistochemistry.
Immunofluorescence:Article Title: Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis.
Article Snippet: Antibody(RRID) Vendor Catalog number Application Working Dilution for WB or (IF/IHC) Rabbit anti-Wilms Tumor1 (AB_2043201) Abcam Cambridge, MA,USA Ab89901 IF (1:200) Rabbit anti-ACTA2 (AB 2721021) ABclonal Shanghai, PRC A7248 IF (1:200) Rabbit anti-Raptor (AB 185538) Bethyl Montgomery,Alabama,USA A300–506A WB,IF 1:3000 (1:200) Rabbit anti-Vimentin (AB 1663663) Bioworld Louis Park, MN,USA BS1491 IF (1:200) Rabbit anti-Rheb (AB 2721022) Cell Signaling Technology Shanghai, PRC 13 879 WB 1:1000 Rabbit anti-Ki67 (AB 2687446) Cell Signaling Technology Shanghai, PRC 9129 IF (1:100) Rabbit anti-β-catenin (AB 331149) Cell Signaling Technology Shanghai, PRC 9562 IF (1:200) Rabbit anti-ZO1 (AB 10898025) Cell Signaling Technology Shanghai, PRC 8193 IF (1:100) Rabbit anti-ZO2 (AB 2203575) Cell Signaling Technology Shanghai, PRC 2847 IF (1:100) Rabbit anti-p-mTOR (AB 330970) Cell Signaling Technology Shanghai, PRC 2971 WB 1:1000 Rabbit anti-mTOR (AB 2105622) Cell Signaling Technology Shanghai, PRC 2983 WB 1:1000 Rabbit anti-p-p70S6K (AB 330944) Cell Signaling Technology Shanghai, PRC 9205 WB 1:2000 Rabbit anti-p70S6K (AB 390722) Cell Signaling Technology Shanghai, PRC 2708 WB 1:1000 Rabbit anti-p-S6 (AB 331679) Cell Signaling Technology Shanghai, PRC 2211 WB 1:3000 Mouse anti-S6 (AB 2238583) Cell Signaling Technology Shanghai, PRC 2317 WB 1:3000 Rabbit anti-ARHGEF4 (AB 2721023) Proteintech Rosemont,IL,USA 55 213–1-AP WB 1:2000 Rabbit anti-Cingulin (AB 10733647) Proteintech Rosemont,IL,USA 21 369–1-AP IF (1:200) Rabbit anti-PRM2 (AB 2721024) Proteintech Rosemont,IL,USA 14 500–1-AP WB 1:1000 Rabbit anti-TNP1 (AB 2206757) Proteintech Rosemont,IL,USA 17 178–1-AP WB 1:1000 Mouse anti-COL1A2 (AB 2716872) Santa Cruz Biotechnolog Dallas, TX,USA SC-393 573 IF (1:100) Mouse anti-Laminin α2 (AB 1125089) Santa Cruz Biotechnolog Dallas, TX,USA SC-55 605 IF (1:100) Mouse anti-β-Actin (AB 476697) Sigma-Aldrich St. Louis, MO,USA A2228 IF (1:200) Mouse anti-α-Tubulin (AB 477582) Sigma-Aldrich St. Louis, MO,USA T6074 IF (1:200) Mouse anti-GAPDH (AB 2721026) Tianjin Sungene Biotech Tianjin, PRC KM9002 WB 1:6000 Lectin PNA, Alexa Fluor TM 488 Conjugate (AB 2315178) Thermo Fisher Scientific L21409 IF 1:100 WB, western blot; IF, immunofluorescence; IHC, immunohistochemistry.
Immunohistochemistry:Article Title: Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis.
Article Snippet: Antibody(RRID) Vendor Catalog number Application Working Dilution for WB or (IF/IHC) Rabbit anti-Wilms Tumor1 (AB_2043201) Abcam Cambridge, MA,USA Ab89901 IF (1:200) Rabbit anti-ACTA2 (AB 2721021) ABclonal Shanghai, PRC A7248 IF (1:200) Rabbit anti-Raptor (AB 185538) Bethyl Montgomery,Alabama,USA A300–506A WB,IF 1:3000 (1:200) Rabbit anti-Vimentin (AB 1663663) Bioworld Louis Park, MN,USA BS1491 IF (1:200) Rabbit anti-Rheb (AB 2721022) Cell Signaling Technology Shanghai, PRC 13 879 WB 1:1000 Rabbit anti-Ki67 (AB 2687446) Cell Signaling Technology Shanghai, PRC 9129 IF (1:100) Rabbit anti-β-catenin (AB 331149) Cell Signaling Technology Shanghai, PRC 9562 IF (1:200) Rabbit anti-ZO1 (AB 10898025) Cell Signaling Technology Shanghai, PRC 8193 IF (1:100) Rabbit anti-ZO2 (AB 2203575) Cell Signaling Technology Shanghai, PRC 2847 IF (1:100) Rabbit anti-p-mTOR (AB 330970) Cell Signaling Technology Shanghai, PRC 2971 WB 1:1000 Rabbit anti-mTOR (AB 2105622) Cell Signaling Technology Shanghai, PRC 2983 WB 1:1000 Rabbit anti-p-p70S6K (AB 330944) Cell Signaling Technology Shanghai, PRC 9205 WB 1:2000 Rabbit anti-p70S6K (AB 390722) Cell Signaling Technology Shanghai, PRC 2708 WB 1:1000 Rabbit anti-p-S6 (AB 331679) Cell Signaling Technology Shanghai, PRC 2211 WB 1:3000 Mouse anti-S6 (AB 2238583) Cell Signaling Technology Shanghai, PRC 2317 WB 1:3000 Rabbit anti-ARHGEF4 (AB 2721023) Proteintech Rosemont,IL,USA 55 213–1-AP WB 1:2000 Rabbit anti-Cingulin (AB 10733647) Proteintech Rosemont,IL,USA 21 369–1-AP IF (1:200) Rabbit anti-PRM2 (AB 2721024) Proteintech Rosemont,IL,USA 14 500–1-AP WB 1:1000 Rabbit anti-TNP1 (AB 2206757) Proteintech Rosemont,IL,USA 17 178–1-AP WB 1:1000 Mouse anti-COL1A2 (AB 2716872) Santa Cruz Biotechnolog Dallas, TX,USA SC-393 573 IF (1:100) Mouse anti-Laminin α2 (AB 1125089) Santa Cruz Biotechnolog Dallas, TX,USA SC-55 605 IF (1:100) Mouse anti-β-Actin (AB 476697) Sigma-Aldrich St. Louis, MO,USA A2228 IF (1:200) Mouse anti-α-Tubulin (AB 477582) Sigma-Aldrich St. Louis, MO,USA T6074 IF (1:200) Mouse anti-GAPDH (AB 2721026) Tianjin Sungene Biotech Tianjin, PRC KM9002 WB 1:6000 Lectin PNA, Alexa Fluor TM 488 Conjugate (AB 2315178) Thermo Fisher Scientific L21409 IF 1:100 WB, western blot; IF, immunofluorescence; IHC, immunohistochemistry.
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